Expression of Immunogenic S1 Gene of Infectious Bronchitis Virus from Field Outbreak in Eukaryotic Cells

Infectious bronchitis (IB) is an acute and contagious disease of poultry. The spike glycoprotein (S) of IB virus is a dimmer and is cleaved into two glycopolypeptides, S1 and S2 post-translationally. S1 gene defines the serotype and plays a major role in induction of protective immunity. Eukaryotic expression systems are frequently employed for the production of recombinant S1 proteins as it is highly glycosylated protein. In present study the S1 gene amplified from isolated field strain of IBV was cloned into eukaryotic expression vector and express in vero cell line. The pQE-TriSystem vector was used as eukaryotic expression vector to express the corresponding protein. The successful expression was confirmed at 24 and 48 hrs post transfection by Reverse Transcriptase-PCR. These promising observations emphasize the need of expression of S1 gene recombinant protein for the development of effective recombinant DNA vaccine against IB in near future.